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human kidney epithelial hek293 cells  (ATCC)


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    ATCC human kidney epithelial hek293 cells
    Human Kidney Epithelial Hek293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 21889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human kidney epithelial hek293 cells/product/ATCC
    Average 99 stars, based on 21889 article reviews
    human kidney epithelial hek293 cells - by Bioz Stars, 2026-06
    99/100 stars

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    p-DNA@SiO 2 particles. (a) TEM images of p-DNA#4 particles with 4.5 µg of plasmid H2B:YFP DNA. (b) Particle diameter (mean ± SD) measured by TEM within a scatter dot plot comparing p-DNA#1-6 ( n = 100). Values for p-DNA#5 and p-DNA#6 are not included due to the presence of aggregates (see Figures S8–S9 ). (*)(**) p-DNA#5-6 not included due to the presence of aggregates (see Figures S5–S6 ). (c) DLS characterization of p-DNA#1-6 bar chart of Z-size (mean ± SD, left Y axis, n = 3) and dot plot in red showing the polydispersity index (PDI) (mean ± SD, right Y axis, n = 3). (d) Flow cytometry quantification of the transfection efficiency (mean fluorescence intensity) of the p-DNA#4 DNA@SiO 2 particles and a representative confocal microscopy image of <t>HEK</t> <t>293</t> cells expressing the recombinant H2B:YFP protein 72 h after transfection. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).
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    p-DNA@SiO 2 particles. (a) TEM images of p-DNA#4 particles with 4.5 µg of plasmid H2B:YFP DNA. (b) Particle diameter (mean ± SD) measured by TEM within a scatter dot plot comparing p-DNA#1-6 ( n = 100). Values for p-DNA#5 and p-DNA#6 are not included due to the presence of aggregates (see Figures S8–S9 ). (*)(**) p-DNA#5-6 not included due to the presence of aggregates (see Figures S5–S6 ). (c) DLS characterization of p-DNA#1-6 bar chart of Z-size (mean ± SD, left Y axis, n = 3) and dot plot in red showing the polydispersity index (PDI) (mean ± SD, right Y axis, n = 3). (d) Flow cytometry quantification of the transfection efficiency (mean fluorescence intensity) of the p-DNA#4 DNA@SiO 2 particles and a representative confocal microscopy image of <t>HEK</t> <t>293</t> cells expressing the recombinant H2B:YFP protein 72 h after transfection. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).
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    p-DNA@SiO 2 particles. (a) TEM images of p-DNA#4 particles with 4.5 µg of plasmid H2B:YFP DNA. (b) Particle diameter (mean ± SD) measured by TEM within a scatter dot plot comparing p-DNA#1-6 ( n = 100). Values for p-DNA#5 and p-DNA#6 are not included due to the presence of aggregates (see Figures S8–S9 ). (*)(**) p-DNA#5-6 not included due to the presence of aggregates (see Figures S5–S6 ). (c) DLS characterization of p-DNA#1-6 bar chart of Z-size (mean ± SD, left Y axis, n = 3) and dot plot in red showing the polydispersity index (PDI) (mean ± SD, right Y axis, n = 3). (d) Flow cytometry quantification of the transfection efficiency (mean fluorescence intensity) of the p-DNA#4 DNA@SiO 2 particles and a representative confocal microscopy image of <t>HEK</t> <t>293</t> cells expressing the recombinant H2B:YFP protein 72 h after transfection. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).
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    p-DNA@SiO 2 particles. (a) TEM images of p-DNA#4 particles with 4.5 µg of plasmid H2B:YFP DNA. (b) Particle diameter (mean ± SD) measured by TEM within a scatter dot plot comparing p-DNA#1-6 ( n = 100). Values for p-DNA#5 and p-DNA#6 are not included due to the presence of aggregates (see Figures S8–S9 ). (*)(**) p-DNA#5-6 not included due to the presence of aggregates (see Figures S5–S6 ). (c) DLS characterization of p-DNA#1-6 bar chart of Z-size (mean ± SD, left Y axis, n = 3) and dot plot in red showing the polydispersity index (PDI) (mean ± SD, right Y axis, n = 3). (d) Flow cytometry quantification of the transfection efficiency (mean fluorescence intensity) of the p-DNA#4 DNA@SiO 2 particles and a representative confocal microscopy image of <t>HEK</t> <t>293</t> cells expressing the recombinant H2B:YFP protein 72 h after transfection. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).
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    p-DNA@SiO 2 particles. (a) TEM images of p-DNA#4 particles with 4.5 µg of plasmid H2B:YFP DNA. (b) Particle diameter (mean ± SD) measured by TEM within a scatter dot plot comparing p-DNA#1-6 ( n = 100). Values for p-DNA#5 and p-DNA#6 are not included due to the presence of aggregates (see Figures S8–S9 ). (*)(**) p-DNA#5-6 not included due to the presence of aggregates (see Figures S5–S6 ). (c) DLS characterization of p-DNA#1-6 bar chart of Z-size (mean ± SD, left Y axis, n = 3) and dot plot in red showing the polydispersity index (PDI) (mean ± SD, right Y axis, n = 3). (d) Flow cytometry quantification of the transfection efficiency (mean fluorescence intensity) of the p-DNA#4 DNA@SiO 2 particles and a representative confocal microscopy image of HEK 293 cells expressing the recombinant H2B:YFP protein 72 h after transfection. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).

    Journal: Drug Delivery

    Article Title: Biodegradable silica nanoparticles for efficient linear DNA gene delivery

    doi: 10.1080/10717544.2024.2385376

    Figure Lengend Snippet: p-DNA@SiO 2 particles. (a) TEM images of p-DNA#4 particles with 4.5 µg of plasmid H2B:YFP DNA. (b) Particle diameter (mean ± SD) measured by TEM within a scatter dot plot comparing p-DNA#1-6 ( n = 100). Values for p-DNA#5 and p-DNA#6 are not included due to the presence of aggregates (see Figures S8–S9 ). (*)(**) p-DNA#5-6 not included due to the presence of aggregates (see Figures S5–S6 ). (c) DLS characterization of p-DNA#1-6 bar chart of Z-size (mean ± SD, left Y axis, n = 3) and dot plot in red showing the polydispersity index (PDI) (mean ± SD, right Y axis, n = 3). (d) Flow cytometry quantification of the transfection efficiency (mean fluorescence intensity) of the p-DNA#4 DNA@SiO 2 particles and a representative confocal microscopy image of HEK 293 cells expressing the recombinant H2B:YFP protein 72 h after transfection. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).

    Article Snippet: HEK 293 (Human Kidney Epithelial Cells, RRID:CVCL_0045) cells were acquired in Innoprot (Innovative Technologies in Biological Systems, Derio, Spain).

    Techniques: Plasmid Preparation, Flow Cytometry, Transfection, Fluorescence, Confocal Microscopy, Expressing, Recombinant

    l-DNA@SiO 2 particles. (a) TEM images of particles l-DNA#4 with 4.5 µg of linear H2B:YFP DNA. (b) Particle diameter (mean ± SD) measured by TEM within a scatter dot plot comparing l-DNA#1-5 ( n = 100). (*) l-DNA#5 is not included due to the presence of aggregates (see Figure S11 ). (c) l-DNA#1-6 bar chart of Z-size (mean ± SD, left Y axis, n = 3) and dot plot in red showing polydispersity index (PDI) (mean ± SD, right Y axis, n = 3). (d) Flow cytometry quantification of the transfection efficiency (mean fluorescence intensity) of the l-DNA#4 DNA@SiO 2 particles and a representative confocal microscopy image of HEK 293 cells expressing the recombinant H2B:YFP protein 72 h after transfection. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).

    Journal: Drug Delivery

    Article Title: Biodegradable silica nanoparticles for efficient linear DNA gene delivery

    doi: 10.1080/10717544.2024.2385376

    Figure Lengend Snippet: l-DNA@SiO 2 particles. (a) TEM images of particles l-DNA#4 with 4.5 µg of linear H2B:YFP DNA. (b) Particle diameter (mean ± SD) measured by TEM within a scatter dot plot comparing l-DNA#1-5 ( n = 100). (*) l-DNA#5 is not included due to the presence of aggregates (see Figure S11 ). (c) l-DNA#1-6 bar chart of Z-size (mean ± SD, left Y axis, n = 3) and dot plot in red showing polydispersity index (PDI) (mean ± SD, right Y axis, n = 3). (d) Flow cytometry quantification of the transfection efficiency (mean fluorescence intensity) of the l-DNA#4 DNA@SiO 2 particles and a representative confocal microscopy image of HEK 293 cells expressing the recombinant H2B:YFP protein 72 h after transfection. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).

    Article Snippet: HEK 293 (Human Kidney Epithelial Cells, RRID:CVCL_0045) cells were acquired in Innoprot (Innovative Technologies in Biological Systems, Derio, Spain).

    Techniques: Flow Cytometry, Transfection, Fluorescence, Confocal Microscopy, Expressing, Recombinant

    Quantification of H2B:YFP protein expression (mean fluorescence intensity) using Lipofectamine TM 2000 and DNA@SiO 2 system with p-DNA and l-DNA in HEK 293 cells. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).

    Journal: Drug Delivery

    Article Title: Biodegradable silica nanoparticles for efficient linear DNA gene delivery

    doi: 10.1080/10717544.2024.2385376

    Figure Lengend Snippet: Quantification of H2B:YFP protein expression (mean fluorescence intensity) using Lipofectamine TM 2000 and DNA@SiO 2 system with p-DNA and l-DNA in HEK 293 cells. Data are shown as the mean ± SD of 3 experimental replicas ( n = 10,000 cells/replica, t- test, * p < 0.05, and *** p < 0.001).

    Article Snippet: HEK 293 (Human Kidney Epithelial Cells, RRID:CVCL_0045) cells were acquired in Innoprot (Innovative Technologies in Biological Systems, Derio, Spain).

    Techniques: Expressing, Fluorescence